RgpB

RgpB belongs to a group of cysteine endoproteases, the gingipains, that are unique to the periodontal pathogen Porphyromonas gingivalis. There are three members to the gingipain family: RgpA and RgpB hydrolyse Arg-Xaa and which Kgp cleaves Lys-Xaa peptide bonds. The cysteine proteinases of the gingipain family are responsible for 85% of the general proteolytic activity generated by this bacterium. The gingipains are potent virulence factors which are able to degrade a broad range of host proteins including extracellular matrix, plasma and immunological proteins. The gingipains are not only responsible for degradation of host proteins, but also possess the potential for the activation of some of them. RgpB have been shown to activate prothrombin to thrombin; prekallikrein – leading to the formation of bradykinin; PAR-1,-2 and -4 receptors located on various cell types including platelets, neutrophils and epithelial cells – leading to platelets aggregation, neutrophils activation, release of pro-inflammatory cytokies.
Product Name Arginine-specific protease Rgp from Porphyromonas gingivalis
Databanks MEROPS classification: clan CD, family C25, peptidase C25.001 / IUBMB: EC 3.4.22.37 (BRENDA) / UniProt: P95493 / GeneBank locus name: PG_0506 / PDB: 1CVR 
Reference Veillard F, Potempa B, Guo Y, Ksiazek M, Sztukowska MN, Houston JA, Koneru L, Nguyen KA, Potempa J. (2015) Purification and characterisation of recombinant His-tagged RgpB gingipain from Porphymonas gingivalis. Biol. Chem. 396:377-384. PMID: 25720118 

Technical information

Source Growth medium of genetically modified P. gingivalis W83 carrying the rgpB gene with an insertion of the hexahistidine coding sequence in the front of the sequence encoding the C-terminal domain.
Purification method Aceton precipitation, DE-52 cellulose ion-exchange chromatography, Ni-Sepharose affinity chromatography
Molecular weight ~48.5 kDa
Amount 50 μg or 100 μg
Purity batch specific (≥80% estimated by SDS-PAGE)
Formulation 20 mM Bis-Tris, 150 mM, NaCl 0.02% NaN3, pH 6.5
Shipping Dry ice
Storage – 80°C. Avoid freeze/thaw cycles by aliquoting protein
Product use All Perio3 Ltd. products are for use only by qualified professionals. All products are for laboratory use in research animals and for in vitro testing. No products are to be used as food or drugs or whatever on or in humans.

 

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